What are the assumptions made during the calculation of net gain of ATP? | NCERT solution | Class 11 Biology | Unit-14 Respiration in Plants



Q.8:- What are the assumptions made during the calculation of net gain of ATP?

 

Answer:-
It is possible to make calculations of the net gain of ATP for every glucose molecule oxidised; but in reality this can remain only a theoretical exercise. These calculations can be made only on certain assumptions that:



  • There is a sequential, orderly pathway functioning, with one substrate forming the next and with glycolysis, TCA cycle and ETS pathway following one after another.
    transferred into the mitochondria and undergoes oxidative phosphorylation.
  • None of the intermediates in the pathway are utilised to synthesise any other compound.
  • Only glucose is being respired – no other alternative substrates are entering in the pathway at any of the intermediary stages.

But these kind of assumptions are not really valid in a living system; all pathway work simultaneously and do not take place one after another; substrates enter the pathways and are withdrawn from it as and when necessary; ATP is utilised as and when needed; enzymatic rates are controlled by multiple means. Hence, there can be a net gain of 36 ATP molecules during aerobic respiration of one molecule of glucose.