Q.4:- RuBisCO is an enzyme that acts both as a carboxylase and oxygenase. Why do you think RuBisCO carries out more carboxylation in C4 plants?
RuBisCO is an enzyme which acts both as carboxylase (carboxylation during photosynthesis) and oxygenase (during photorespiration). But RuBisCO carries out more carboxylation in C4 plants. In C4 plants, initial fixation of carbon dioxide occurs in mesophyll cells. The primary acceptor of C02 is phosphoenol pyruvate or PER It combines with carbon dioxide in the presence of PEP carboxylase or PEPcase to form oxaloacetic acid or oxaloacetate. Malic acid or aspartic acid is translocated to bundle sheath cells through plasmodesmata. Inside the bundle sheath cells they are decarboxylated (and deaminated in case of aspartic acid) to form pyruvate and CO2 . CO2 is again fixed inside the bundle sheath Cells through Calvin cycle. RuBP of Calvin cycle is called secondary or final acceptor of CO2 in C4 plants. Pyruvate is sent back to mesophyll cells.